No More Microinjection, VisuFect® 100 Gene Delivery Reagent

	비싸고 어려운 Microinjection은 이제 그만!!! Transfection 효율 걱정도 그만!!
Figure 1. VisuFect® 100 results from various cell types. VisuFect® 100 emitted red light. Red means that VisuFect® 100 with oligonucleotides enter the cells.
● Application   - Transfer short oligonucleotides (e.g. DNA, RNA, siRNA, miRNA) - Transfection of primary difficult-to-transfect cells, including human embryonic stem (ES) cells, human fibroblast cells - In vivo gene delivery zygote, egg and sperm (e.g. zebrafish, xenopus, porcine etc) - Development study - Gene delivery study according time lap - HTS study (high-throughput)
● Protocol for Treatment of the VisuFect® 100 for Zygote   A. Prepare VisuFect® 100 stock (100pmole/ul). B. Drop VisuFect® 100 stock (to be final conc. 1 pmole/ul)* into culture medium which contains Zygote or Sperm. C. Mix plate gently. D. Incubate for 12-16 hrs at CO2 incubator. E. Wash for 3 times and change to fresh culture medium. F. Visualize transfected cell with a confocal microscopy. (Ex.675nm / Em.694nm) * It is necessary to optimize VisuFect® 100 concentration for customer’s experiments
● Reference   Jin Young Joo, Jonghwan Lee, Hae Young Ko, et al., Microinfection free delivery of miRNA inhibitor into zygotes, Scientific Reports(2014) 4:5417