No More Microinjection, VisuFect® 100 Gene Delivery Reagent

	비싸고 어려운 Microinjection은 이제 그만!!! Transfection 효율 걱정도 그만!!
Figure 1. VisuFect® 100 results from various cell types. VisuFect® 100 emitted red light. Red means that VisuFect® 100 with oligonucleotides enter the cells.
● Application   - Transfer short oligonucleotides (e.g. DNA, RNA, siRNA, miRNA) - Transfection of primary difficult-to-transfect cells, including human embryonic stem (ES) cells, human fibroblast cells - In vivo gene delivery zygote, egg and sperm (e.g. zebrafish, xenopus, porcine etc) - Development study - Gene delivery study according time lap - HTS study (high-throughput)
● Protocol for Treatment of the VisuFect® 100 for Zygote   A. Prepare VisuFect® 100 stock (100pmole/ul). B. Drop VisuFect® 100 stock (to be final conc. 1 pmole/ul)* into culture medium which contains Zygote or Sperm. C. Mix plate gently. D. Incubate for 12-16 hrs at CO2 incubator. E. Wash for 3 times and change to fresh culture medium. F. Visualize transfected cell with a confocal microscopy. (Ex.675nm / Em.694nm) * It is necessary to optimize VisuFect® 100 concentration for customer’s experiments
● Reference   Jin Young Joo, Jonghwan Lee, Hae Young Ko, et al., Microinfection free delivery of miRNA inhibitor into zygotes, Scientific Reports(2014) 4:5417

[Any] Lab Basic and Essential Equipment - Shaker, Vortex(mixer), Pipette Aid

		-Maximum shaking weight of 3kg -Compact space-saving design fits easily in  the incubator and refrigerator -Continuous operation, speed range of 0-200 rpm Cat No. Description ANY-L-S Any Classic Linear Shaker ANY-O-S Any Classic Orbital Shaker
		-Maximum shaking weight of 3kg -Compact space-saving design fits easily in the incubator and cryogenic box -Aluminium-alloyed platform provides strong bearing capability, with non-slip mat holds vessels prevent slipping -Continuous operation,speed range of 0-80   rpm see-saw wave, angle 7° Cat No. Description ANY-R-S Any Classic Rocking Shaker
			-Maximum shaking weight of 3kg -Compact space-saving design fits easily in the incubator and cryogenic box -Aluminium-alloyed platform provides strong bearing capability, with non-slip mat holds vessels prevent slipping -Continuous operation,speed range of 0-80 rpm Angle7° Cat No. Description ANY-3D-S Any Classic 3D Shaker
		-Touch and continuous operations -Wide speed range of 0-2500rpm, stepless  speed regulation -Various mixing applications with optional adapters, handles up to 48 tubes -Specially designed vacuum suction feet hold  to the work surface and prevent the unit from movement -Aluminium cast base avoids unnecessary “walking” Cat No. Description ANY-V-M Any Vortex Mixer (Standard top cup)
별도 구매가능한 Accessories
	-Full volume range of 0.1-100ml -Easy to one-handed operation -Light weight, ergonomic designs -Efficient lithium-ion battery offers long runtime on each charge -Low battery alert -Powerful motor fills a 25ml pipette in＜5 seconds -Supplied with integral but replaceable 0.45um filter -Can be used while charging -Large LCD display provides visual confirmation of remaining battery charge and speed settings Cat No. Description ANY-P-A Any Pipette Aid, Euro plug

http://www.seoulin.co.kr

VisuFect® 100 Gene Delivery Reagent

√ High Efficiency                                       √ Simple and Fast                                       √ Easy Monitoring                                       √ Low Cytotoxicity                                       √ Ready-to-Use
VisuFect® 100 Gene Delivery Reagent
Description   VisuFect® 100 is made up of an organic compound and chemically for the uptake by cells without a transfection reagent. VisuFect® 100 can deliver oligonucleotides in which a user id interested such as DNA aptamers, siRNAs, miRNAs or antisense RNAs into cell without any transfection reagent. Since VisuFect® 100 contains fluorophore, the researcher can easily monitor efficiency of transfection. VisuFect® 100 is a customized product which made by customer’s own sequence. VisuFect® 100 reagent is ideal for transfection of various cells, including primary difficult-to-transfect cells zygote, egg and sperm. Transfection can also be performed regardless of the presence or absence of serum. VisuFect® 100 demonstrates superior transfection efficiency and minimal toxicity compared with the lipid-based or other polymer-based transfection reagents.
Figure 1. VisuFect® 100 results from various cell types. VisuFect® 100 emitted red light. Red means that VisuFect® 100 with oligonucleotides enter the cells.
Application   ● Transfer short oligonucleotides (e.g. DNA, RNA, siRNA, miRNA) ● Transfection of primary difficult-to-transfect cells, including human embryonic stem (ES) cells, human fibroblast cells ● In vivo gene delivery zygote, egg and sperm (e.g. zebrafish, xenopus, porcine etc) ● Development study ● Gene delivery study according time lap ● HTS study (high-throughput)
Feature   ● Organic compound, hydrophilic and fluorescence-label ● Chemically modified reagent for gene delivery (Clathrin-mediated internalization) ● Customized product which made by customer’s own sequence ● Source : 10-30 mer oligonucleotide            DNA aptamers, siRNAs, miRNAs or antisense RNAs, antisense oligonucleotides ● Easy monitoring gene delivery efficiency : fluorescence-label (Figure 2) ● Fast and simple gene delivery with no need to change media ● High efficiency regardless of the presence or absence of serum ● Low cytotoxicity (Figure 3) ● Easy to handle : Ready-To-Use, Customized synthetic product
Figure 2. The confocal microscopy result after treatment of VisuFect® 100 to porcine eggs (for 18h) and sperm (for 2h). VisuFect® 100 was diluted with medium (that was) used for porcine cell culture. VisuFect® 100 emitted 694nm waves and red light.
Figure 3. Low cytotoxicity of VisuFect® 100. A comparison of development rate of blastocyst between VisuFect® 100 treatment and microinjection using by same siRNA. It shows almost same development rate between control and VisuFect® 100 samples. However, in the case of microinjection, the development rate of blastocyst was reduced.
Reference   Jin Young Joo, Jonghwan Lee, Hae Young Ko, et al., Microinfection free delivery of miRNA inhibitor into zygotes, Scientific Reports(2014) 4:5417
Ordering Guide   Product Cat. No Size VisuFect® 100 SLVF100-10 10 Reactions VisuFect® 100 SLVF100-25 25 Reactions VisuFect® 100 Positive (GAPDH) Control Kit Request 　 VisuFect® 100 Negative Control Kit Request 　 VisuFect® 100 Control Kit Request 　 VisuFect® 100 Start Kit Request